Immunoenzymatic colorimetric method for quantitative determination of thyroxine (T4) concentration in human serum and plasma.
T4 ELISA kit is intended for laboratory use only.
ANA 12 Line Dot
ANA 12 Line Dot
ANA 12 LINE is used for the separate qualitative determination of auto-antibodies to nuclear and cytoplasmic antigens (dsDNA, nucleosomes, Sm, ribosomes, histones, RNP, SS-A 60 kDa, SS-A 52 kDA, SS-B, Scl-70, CENP-B and Jo-1) in human serum or plasma.
pANCA IFA is used for the sensitive qualitative and semiquantitative
determination of IgG antibodies to neutrophil
cytoplasmatic antigens (ANCA) in human serum using indirect
immunoflorescence assay on formalin fixed human granulocytes
for the differential diagnosis of systemic vasculitis (SV). The assay
is used for the confirmation of pANCA positive results found on
ethanol fixed granulocytes.
Anti-MuSK IFA is a reagent set for the qualitative and semi-quantitative measurement of antibodies against the MuSK antigen in human serum. For this HEp-2 cells, which have been transfected with MuSK antigens.
Anti-huTransG is used for the quantitative or semi-quantitative
determination of IgA autoantibodies to tissue transglutaminase
(tTG, transglutaminase 2) in human serum or plasma for the
differential diagnosis of celiac disease.
Coxsackieviruses are nonenveloped, icosahedral, single
stranded RNA (+) viruses of small size (28-30 nm) included in
the genus Enterovirus. Enteroviruses are responsible for a wide
array of clinical diseases, including aseptic meningitis,
encephalitis, myocarditis and pericarditis, respiratory disease
or hand-foot-and-mouth disease.
Dengue (DF) and dengue hemorrhagic fever (DHF) are caused by one of
four closely related, but antigenically distinct, virus serotypes (DEN-1,
DEN-2, DEN-3, and DEN-4), of the genus Flavivirus. DF and DHF are
primarily diseases of tropical and sub tropical areas, and the four different
dengue serotypes are maintained in a cycle that involves humans and the
Aedes mosquito. Infections produce a spectrum of clinical illness ranging
from a nonspecific viral syndrome to severe and fatal hemorrhagic disease.
Clinical manifestations include rash, sudden onset of fever, chills, severe
headache, nausea, myalgias and arthralgias, leukopenia, thrombocytopenia
and hemorrhagic manifestations. It occasionally produces shock and
hemorrhage, leading to death. Important risk factors for DHF include the
strain of the infecting virus, as well as the age, and especially the prior
dengue infection history of the patient.
Dengue viraemia appears to be universal in febrile patients with dengue; it
occurs prior to the onset of fever and symptoms and peaks 2–3 days after
the onset of illness. A diagnosis of acute infection with dengue virus can
be made by isolating the virus or by detecting viral genome or antigen.
Serologically, a primary infection with dengue virus results in detectable
levels of IgM antibodies by the third afebrile day after infection. These
IgM antibodies persist for 1–2 months after infection. IgG antibodies are
detected approximately 14 days after onset of primary infections.
Secondary infections with dengue virus are characterized by a rapid
increase in IgG antibody levels. Owing to the relatively late increase in
antibody levels to a concentration that can be detected diagnostically, a
negative result for an antibody test early in the course of disease is not
definitive. Specimens should be collected at least 7 days after the onset of
symptoms in order to rule out the possibility of an acute infection with
Serology is the most widely applied method used in routine diagnosis.
Traditionally, hemagglutination inhibition and virus neutralization tests
have been used. At present ELISAs for IgM and IgG antibodies are the
standards for the serological analysis of dengue virus infections, as they
are simple and allow large numbers of samples to be tested.
Mump virus is the ethiological agent of the mump. Mumps is an illness
characterized by parotid swelling and usually accompanied by generalized
symptoms. It is one of the most common causes of aseptic meningitis.
Other common symptoms are orchitis and inflammation of pancreas and
ovaries. Some of the mumps infections are subclinical or unrecognized and
may require viral isolation and/or some other serological procedure.
Current methods for serodiagnosis of mumps infection are serum
neutralization, hemagglutination-inhibition, immunofluorescence,
complement fixation and ELISA tests. ELISA is as sensitive as the
neutralization test and more sensitive than hemagglutination-inhibition and
complement fixation. An increase in IgG titer is helpful, specially if can be
shown to be greater than that against any parainfluenza virus. The IgM
response is also useful, althouhg it may be suppressed in cases that occur
after prior sensibilization. It can also be falsely positive as a result of
concurrent parainfluenza virus infection.
Toxoplasma gondii is a protozoan that presents three stages in
its developmental life cycle: oocyst, tachyzoite and bradyzoite.
While only members of the family Felidae can be definitive
hosts of the parasite, a great variety of animals can harbour
the tissue cysts. Although consuming contaminated food is the
most common way of becoming infected, transplacental
infection may occur. The disease is normally benign, but
central nervous system disease may appear in
immunocompromised patients and the newborn.
Direct agglutination, Rose Bengal test, Coombs´test and ELISA are the
most widely used techniques for the serologic diagnosis of brucellosis.
Detection of IgG against Brucella lipopolysaccharide (LPS) is suitable for
the diagnosis of all forms of the disease. The ELISA technique is sensitive
and specific for the detection of IgG antibodies against Brucella.
Vibrio cholerae is a Gram-negative, comma-shaped, flagellate,
facultative anaerobic bacterium. Its natural habit is aquatic,
frequently associated with zooplancton, shellfish or aquatic
plants. Cholera disease is caused by toxigenic strains of
serogroups O1 and O139. People are infected by drinking
water or eating food contaminated with the cholera bacterium.
The infection is often mild or without symptoms, but
sometimes it can be severe, with watery diarrhea and vomiting
that can lead to rapid dehydration and electrolyte imbalance.